Shiv Om Pratap, Ruchi Gaur, Sandeep Kumar Verma, Suryakant Mishra
The current study involves the utilization of fifteen polymorphic microsatellite markers that represent various chromosomal regions of the chicken genome. Current study was carried out to assess the genetic diversity upon the basis of intra and inter-breed molecular variations between two selected diverse chicken populations: Kalamasi (KN) and the well-known Italian layer breed, White Leghorn (WLH) reared from last 30 generations in CARI, India. The objective of this study remain to proven the efficacy of molecular marker system to reflect the level of molecular identity in contrast to manual data analysis among these two selected breeds having different breeding histories. Kalamasi (KN) has reared without any scientific breeding program as natural conservation program especially in villages by poor tribals and villagers with undiluted germ-plasm with unique alleles. While the other commercial layer type WLH experienced long-term selection for higher egg production from several generation by ex-situ conservation. The selected panel of fifteen polymorphic microsatellite molecular markers (STRs) that represent various chromosomal regions of the chicken genome for identifying unique alleles among the selected chicken breeds by having distinct breeding histories. The KN breed is remained under ex-situ conservation while the WLH underwent long-term selection for high egg number.
